用EST-SSR分子标记技术构建大白菜核心种质及其指纹图谱库

Construction Chinese Cabbage (Brassica rapa L.) Core Collection and its EST-SSR Fingerprint Database by EST-SSR Molecular Markers

利用EST-SSR分子标记对大白菜种质资源基因库中686份样品所代表的1900份大白菜种质资源进行分析研究。构建大白菜种质资源的核心种质并且形成核心种质的EST-SSR指纹图谱库。结果表明利用4组鉴定白菜品种的EST-SSR的特异性标记组合,获得近158个EST-SSR多态的标记,对大白菜种质资源基因库中686份样品所代表的1900份大白菜种质资源进行核心种质的构建提供了分析数据。形成的核心种质包括168份样品,占库存资源的8.8%,它的多态位点百分率保持了原群体的100%。所构建的核心种质涵盖了原资源的绝大部分区域来源的品种,包含了早、中、晚熟品种中所有典型的大白菜类型和其相关的特征特性。并进一步进行了核心种质资源遗传多样性分析。核心种质的EST-SSR指纹图谱库中,每一份样品的指纹都是唯一的,为登记、评价、整理、分发、繁殖等种质资源库的管理和育种者对其材料的利用提供了重要的有价值的信息。EST-SSR标记组合是构建中国大白菜核心种质及其指纹图谱的经济、高效的方法。

Applying four sets of primer combinations to analyze the 686 Chinese cabbage accessions which originate from 1 900 accessions. Constructed Chinese cabbage germplasm resource core collections and form its EST-SSR fingerprint database. Four primer combinations constituted by thirteen EST-SSR primer pairs are applied to screen 686 Chinese cabbages （Brassica rapa L.） accessions, and 158 alleles are generated. The core collection is composed of 168 accessions which only represents 8.8% of the 1 900 original germplasm resource, but has 100% polymorphism. The core collection contains typical sorts of varieties with early, middle and late autumn growing habits from the most ecological regions. The EST-SSR fingerprint database constructed for the core collection includes information for every accession, which will be valuable for variety breeding and gene bank organization. EST-SSR marker combination are valuable and informative to construct Chinese cabbage core collection and fingerprint database.