DNA提取方法对一串红不同部位DNA提取的比较

Comparison of the DNA Extraction Methods on the Quality of DNA Extracted from Different Parts of Salvia splendens

本文以一串红的叶片、茎段、花为材料,分别采用高盐低pH法、十二烷基硫酸钠(SDS)法、改良十六烷基三乙基溴化铵(CTAB)法、核DNA法和十六烷基三乙基溴化铵(CTAB)区室法等5种不同方法对其进行了基因组DNA提取效果的比较分析。结果表明,除用高盐低pH法在一串红花中未提取出DNA外,其它均可提取出DNA,都能进行RAPD扩增。5种方法在一串红DNA提取中,提取效果由高到低依次为:核DNA法、改良CTAB法、SDS法、CTAB区室法、高盐低pH法;3个部位提取结果显示:叶片较容易得到高质量的DNA,茎段次之,花最差。

Experiment was conducted with leaves, stems and flowers of Salvia splendens and 5 methods, including high salt and low pH, SDS, modified CTAB, nuclear DNA and CTAB subarea methods were used for genome DNA extraction for studying their effects on DNA extraction. The results showed that all of these DNA extraction methods can obtain DNA and can be amplified by RAPD, with one exception that DNA can not be obtained from flowers of Salvia splendens by high salt low pH method. The extraction results in descending order in five methods tested were： nuclear DNA, modified CTAB, SDS, CTAB subarea, high salt and low pH. And high quality of DNA extracted from leaves of Salvia sple nde ns was easier than that from stems with flowers the worst.