猪胞浆内单精子注射研究进展

Advances in Porcine Intracytoplasmic Sperm Injection

胞浆内单精子注射将体外受精和显微操作技术相结合,已成功应用于哺乳动物受精机理研究,家畜性控胚胎生产,动物转基因等领域。综述了猪胞浆内单精子注射的主要影响因素,包括卵母细胞体外成熟和预处理、精子的选择和处理、注射卵的人工激活以及操作技术的改进,为进一步提高猪胞浆内单精子注射的效率以及该技术的应用提供参考。

Intracytoplasmie sperm injection, which combined with in vitro fertilization and micromanipulation techniques, has been applied to research the molecular mechanisms of fertilization, and produce the sexing livestock and transgenie animals. The research advances in porcine intracytoplasmic sperm injection, including in vitro maturation and pretreatment of oocytes, selection and treatment of spermatozoon, artificial activation of injected oocytes after injection, and improvement of the operation technique were reviewed.