摘要
目的比较人髓系白血病细胞株HL-60细胞和耐阿霉素的细胞(HL-60/ADR)的整体蛋白表达谱,以期获得人髓系白血病细胞的耐药相关蛋白。方法提取HL-60细胞和HL-60/ADR细胞的总蛋白,采用荧光差异显示的双向凝胶电泳技术(DIGE)比较分析差异表达蛋白;经胶内酶解,MALDI-TOF/TOF质谱分析,搜索蛋白质数据库,获得差异蛋白质的信息。结果经过初步筛选和质谱分析共获得16个具有统计学意义的表达差异蛋白点,其中13个在HL-60细胞中表达上调,3个表达下调。主要是代谢酶类、DNA修复、信号转导相关蛋白、细胞周期调节蛋白和细胞增殖与凋亡等相关的蛋白。结论根据人髓系白血病细胞株HL-60细胞和HL-60/ADR细胞的差异蛋白表达谱比较,筛选出耐药相关蛋白。
Objective To compare the protein expression profiles between human myeloid leukemia HL-60 cells and adriamycin-resistant HL-60 cells and to explore drug resistance-associated proteins in myeloid leukemia. Methods Total cellular proteins extracted from HL-60 and adriamycin-resistant HL-60 cells were separated by two dimensional differences in gel electrophoresis （2D DIGE）. Differe- nialtly expressed proteins were analyzed by mass spectrometry （MALDI-TOF/TOF）, and protein database searching. Results Sixteen significantly different protein expression spots were screened, among which thirteen protein spots were identified to be up-regulated and three down-regulated in adriamycin-resistant HL-60 cells. The identified proteins were metabolic enzymes, DNA repairs, proteins related with signal transduction, cell cycle regulator, and cellular proliferation and apoptosis proteins. Conclusion 2D DIGE is a useful approach to investigate different protein expression-related treatment of resistance and could provide a novel clue of the resistant mechanisms in myeloid leukemia.
出处
《福建医科大学学报》
2009年第1期1-4,共4页
Journal of Fujian Medical University
基金
国家自然科学基金(30572132)
福建省自然科学基金(C0410029)
福建医科大学基金(FJGXY04022)
