石油降解菌多食鞘氨醇杆菌的发酵条件优化

Optimization of the Liquid Fermentation Condition of the Petroleum Degradation Bacteria Sphingobacterium multivorum

多食鞘氨醇杆菌SWH-2是一株对石油具有较高降解活性的细菌,笔者对其产菌的发酵条件进行了初步的优化。通过单因素筛选和均匀设计优化得到发酵的最适培养基为：MgSO4.7H2O 0.5%,NaCl 0.5%,3%豆粕粉。最佳发酵条件为：初始pH7.0-7.2,发酵温度25℃,摇床转速220r/min,发酵时间21-22h。按此优化条件进行试验,获得了635×10^8cfu/mL的活菌体数。图4、表5、参10。

The bacteria Sphingobacterium multivorum SWH- 2 has the better effect to the degradation of crude oil. And the fermentation condition of it was primary optimized. By the way of single element screen and uniform design,we got the optimum media is MgSO4·7H2O 0.5% ,NaCl 0.5% and 3% powder of soybean dregs, with initial pH 7.0- 7.2,culturing at 25℃ ,220r/min,the time of fermentation is 21 - 22h.The coneentration is 635 × 10Scfu/mL. Fig.4,Tab.5,Ref10.