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DNA-蛋白质相互作用研究的方法及其新进展 被引量：5

Advances in the Techniques for Studying the DNA-protein Interactions
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摘要 DNA与蛋白质的相互作用参与生命体内的许多生物学过程,关于二者相互作用的研究是人们了解基因表达机制、揭开生命奥秘的关键所在。该文简要阐述了传统研究DNA-蛋白质相互作用的常用方法及其优缺点,并综述了近年来该领域所采用的新技术及其新进展。 Protein- DNA interactions participate in many biological processes. It plays a key role in knowing the truth of genome regulation and unravelling the mysteries of life. In the present paper, the classical methods and their advantages and disadvantages for investigation the interactions of DNA - protein have been reviewed. Meanwhile, the latest advances in the new techniques for studying protein - DNA interactions were described, and the perspectives on the future development were also propesed.
作者 蔡容华 李强 张建军 邢佩佩 施碧红
机构地区 福建师范大学教育部工业微生物工程研究中心
出处 《生物技术》 CAS CSCD 北大核心 2009年第1期93-97,F0004,共6页 Biotechnology
基金 福建省自然科学基金项目资助(No.B0610027)
关键词 相互作用 DNA 蛋白质 研究技术 interactions DNA protein techniques
分类号 Q81 [生物学—生物工程]
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参考文献38

  • 1Gustafsdottir SM, Schlingemann J, Rada - Iglesias A, et al. In vitro analysis of DNA - protein interactions by proximity ligation[J]. Proc Natl Acad Sci USA, 21307,104: 3067 - 3072.
  • 2祁小廷,柴小清,刘靖,柴团耀.改造地高辛标记DNA和检测试剂盒用于凝胶阻滞实验的新方法[J].遗传,2006,28(6):721-725. 被引量：6
  • 3Sandaltzopcules R, Becker P B. Solid phase DNase Ⅰ footprinting: quick and versatile[J]. Nucleic Acids Res, 1994,22(8) : 1511 - 1512.
  • 4Li J J, Herskowitz I. Isolation of ORC6, a component of the yeast origin recognition complex by a one - hybrid system[J]. Science, 1993, 262:1870 - 1874.
  • 5Qin F,H J,Zhang G Y,et al. Isolation and Structural Analysis of DRE- Binding Transcription Factor from Maize ( Zea mays L. ) [ J ]. Acta Botanica Sinica,2003,45 (3) :82.
  • 6Mueller P R, Wold Barbara. In vivo footpringting of a muscle specific enhancer by ligation mediated PCR[J]. Science, 1989,246:780- 786.
  • 7Lis J, Wu C. Protein traffic on the heat shock promoter; Parking, stalling, and trucking along[J]. Cell, 1993,74:1 - 4.
  • 8Ira H, Grass JA, Johnson KD, et al. Measurement of protein - DNA interactions in vivo by chromatin immunoprecipitation [ J ]. Methods Mol. Biol., 2004,284:129- 146.
  • 9Weinmann AS, Famham PJ. Identification of unknown target genes of human transcription factors using chromatin immunoprecipitation[J]. Methods,2002, 26:37 - 47.
  • 10Zella LA, Kim S, Shevde NK, et al. Enhancers located within two introns of the vitamin D receptor gene mediate transcriptional autoregulafion by 1, 25 - dihydroxyvitamin D3[J] .Mol Endocrinol,2006,20:1231 - 1247.

二级参考文献18

  • 1[1]Nylander C,Liedberg B,Lind T.Sensorsand Actuators,1982,3:79～88
  • 2[3]S.Lowry,K.Nishikida,E.Y.Jiang,A.W.Wark,V.Kodoyianni,S.C.Weibel,and T.Burland,Thermo Electron Application Note 50958,Madison,WI,USA,2005
  • 3[4]R.M.Corn.and S.C.Weibel,Handbook of Vibrational Spectroscopy,Eds.,J.M Chalmers,P.R.Griffith,John Wiley & Sons,Ltd.,Chichester,UK,,2001,2:1057
  • 4[5]E.Y.Jiang,S.Lowry,K.Nishikida,V.Kodoyianni,and S.C.Weibel,Thermo Electron Application Note 50946,Madison,WI,USA,2005
  • 5[6]G.J.Wegner,H.Lee and R.M.Corn,Anal.Chem.,2002,74:5161～5168
  • 6[7]E.Y.Jiang,A.W.Wark,S.C.Weibel,Thermo Electron Application Note 50947,Madison,WI,USA,2005
  • 7Meyer RR,Laine PS.The single-stranded DNA-binding protein of Escherichia coli.Microbiol Rev,1990,54(4):342～380
  • 8Sancar A,Williams KR,Chase JW,Rupp WD.Sequences of the ssb gene and protein.Proc Natl Acad Sci USA,1981,78(7):4274～4278
  • 9Lohman TM,Ferrari ME.Escherichia coli single-stranded DNA-binding protein:multiple DNA-binding modes and cooperativities.Annu Rev Biochem,1994,63:527～570
  • 10Witte G,Urbanke C,Curth U.DNA polymerase Ⅲ chi subunit ties single-stranded DNA binding protein to the bacterial replication machinery.Nucleic Acids Res,2003,31 (15):4434～4440

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生物技术
2009年 第1期

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