微柱凝胶抗人球蛋白试验检测孕妇抗A(B)IgG抗体早期诊断ABO-HDN的研究

Determination of Anti-AB IgG in Pregnant Women by Microcolumn gel Antiglobin Test for Early Diagnosis of ABO Neonatal Haemolysis

[目的]运用微柱凝胶抗人球蛋白试验检测孕妇抗A(B)IgG抗体效价,以预防ABO新生儿溶血病的发生。[方法]采用微柱凝胶抗人球蛋白试验检测抗IgGA(B)抗体效价,统计其与ABO新生儿溶血病发生率的关系。[结果]356例O型血型孕妇中,238例IgG抗A(B)抗体效价≤64时,均未发生ABO-HDN;IgG抗A(B)抗体效价为128、256、512时,ABO-HDN发病率分别为7.8%、17.6%、2/3和4.6%、7.1%、1/2。[结论]微柱凝胶抗人球蛋白试验是一种简便、快速、灵敏、准确的试验方法;孕妇血清中IgG抗A(B)抗体效价的检测有助于早期诊断、预防ABO-HDN。

[Objective]To determine the level of anti-A/B IgG in pregnant women by using microcolumn gel antiglobin test ,to prevent the occurrence of ABO neonatal haemolysis. [Methods]The titers of serum anti-A/B IgG antibody were determined by microcolumn gel antiglobin test,and their correlation with ABO neonatal haemolysis was evaluated. [Results] There were 238 in the 356 with O group blood- whose serum anti-A/B IgG less than 64, ABO neonatal haemolysis were not happened;Otherwise,the incidence of ABO-HDN were 7.8% ,17.6% ,2/3 and 4.6% ,7.1% ,1/2 in pregnant women when the titer of serum anti-A/B IgG were 128,256,512 respectively. [Conclusion] Microcolumn gel antiglobin test is a simple, rapid, sensitive and accurate method; the determination of the titers of serum anti-A/B IgG in the pregnant women could have great clinic value in early diagnosis and prevention of the ABO neonatal haemolysis.