非小细胞肺癌组织HIF-1α和CAIX及VEGF表达临床意义的探讨

Expressions of HIF-1α,carbonic anhydrase Ⅸ and VEGF in non-small cell lung carcinoma and their clincical significance

目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)病理组织中乏氧诱导因子-1α(hypoxia inducible factor 1α,HIF-1α)、碳酸酐酶9(carbonic anhydraseⅨ,CAⅨ)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达水平及其临床意义。方法:免疫组化方法测定59例NSCLC和14例肺部良性病变病理切片中CAⅨ、HIF-1α和VEGF的表达,并与患者的临床资料进行统计分析。结果:59例肺癌组织中27例(45.8%)有HIF-1α表达,44例(74.6%)有CAⅨ表达,43例(72.9%)有VEGF表达。CAⅨ、HIF-1α和VEGF的表达在不同临床分期、不同瘤体大小差异均无统计学意义;CAⅨ的表达在不同病理类型差异有统计学意义,鳞癌中CAⅨ的表达显著高于腺癌,P=0.039;HIF-1α的表达与组织分化程度呈负相关,P=0.015;VEGF的表达在淋巴结转移阳性组较阴性组高,P=0.031。高HIF-1α表达与高CAⅨ表达有显著相关性,P=0.004,其表达区域重叠。同时,HIF-1α和VEGF的阳性表达相关,P=0.011。结论:HIF-1α和CAⅨ均在NSCLC中高水平表达,均有可能影响NSCLC的生物学行为和预后。

OBJECTIVE： To investigate the correlation between the expressions of hypoxia inducible factor 1α （HIF-1α）, carbonic anhydrase Ⅸ（CA Ⅸ） and vascular endothelial growth factor （VEGF） in human non small cell lung cancer and their clinical significance. METHODS： Immunohistochemistry was used to detect the expressions of CAⅨ, HIF-1α and VEGF in pathological tissue sections and the correlation of them with the age, sex, size of tumor, histopatho logical type,differentiation,stage and lymph node metastasis were an alyzed statistically. RESULLTS： HIF-1α was expressed in 27 eases （45.8%）,CAIX in 44 cases （74.6%）, and VEGF in 43 cases （72. 9%）. There was no significant difference among stages and tumor sizes. The expression of CAⅨ among the patients with differ ent histopathological types were different significantly, P = 0. 039. The expression of HIF-1α among the patients with different differen ciations were different significantly, P=0. 015. The expressions of VEGF between the patients with or without lymoh node metastasis were significantly different, P = 0.031. HIF-1α expression was strongly correlated with CAⅨ and co-localized with CAⅨ in corre sponding areas, P=0. 004. HIF-1α expression was also significantly correlated with VEGF, P=0. 011. CONCLUSION：The high expressions of CA Ⅸ and HIF-1α occurre in most of cases of NSCLC, which may play a role in the biologic behavior and prognosis of NSCLC.