食管鳞癌组织PPARγ和MMP-7蛋白表达临床意义的探讨

Expressions of PPARγ and MMP-7 in esophageal squamous cell carcinoma and their significance

目的:探讨过氧化物酶增殖物激活受体γ(peroxisome proliferators-activated recep-torγ,PPARγ)和基质金属蛋白酶-7(matrix Metallo-proteinase-7,MMP-7)在食管鳞癌组织中的表达及其生物学意义。方法:免疫组织化学SP法检测PPARγ和MMP-7蛋白在40例食管鳞状细胞癌、25例非典型增生和13例正常黏膜组织中的表达。流式细胞术检测其中30例食管鳞癌、15例非典型增生和10例正常黏膜组织中PPARγ和MMP-7蛋白含量。结果:PPARγ在癌组织中的阳性表达率为52.5%(21/40),高于不典型增生组织(20.0%,5/25)和正常黏膜组织(7.7%,1/13),P<0.05;MMP-7在癌组织中的阳性表达率为65.0%(26/40),高于不典型增生组织(28.0%,7/25)和正常黏膜组织(0),P<0.05。流式细胞术结果与免疫组化结果一致。PPARγ的表达与细胞分化程度、淋巴结转移相关,P<0.05;MMP-7的表达与浸润深度和淋巴结转移相关,P<0.05;PPARγ和MMP-7的表达成正相关。结论:PPARγ和MMP-7的表达与食管鳞癌的发生发展密切相关,且两者在此过程中可能具有协同作用。

OBJECTIVE： To investigate the expressions and biological significance of PPARγ and MMP-7 in esopha geal squamous carcinoma tissnes. METHODS： The expressions of PPARγ and MMP-7 were examined by SP immunohistochemical stain （IHC） in 40 cases of esophageal carcino ma specimens, 25 cases of dysplasia and 13 cases of normal tissues. Moreover, they were examined in 30 cases of esophageal carcinoma specimens, 15 cases of dysplasia and 10 cases of normal tissues by FCM. RESULTS： The positive expression rate of PPAR7 in esophageal carcinoma （52. 5%, 21/40） was sig nificantly higher than that in dysplasia （20. 0%, 5/25） and normal tissues （7.7%,1/13）, P〈0. 05, and high expression of PPARy was correlated with the tumor differentiation and metastasis of esophageal squamous cell carcicoma,P〈0. 05. The positive expres sion of MMP-7 in esophageal carcinoma （65.0%, 26/40） was significantly higher than that in dysplasia （28. 0%, 7/25） and normal tissues （0） ,P〈0. 05, and high expression of MMP-7 was correla ted with the invasion and metastasis of esophageal squamous cell carcinoma, P〈0.05. There was a negative correlation between MMP-7 and PPARγ. CONCLUSION： PPARγ and MMP-7 can be involved in carcinogenesis and progression of esophageal squamous cell carcinoma.