结直肠癌组织趋化因子受体CXCR4表达及其与淋巴结和肝转移相关性的探讨

Correlation of CXCR4 expressions with lymphatic and hepatic metastasis in colorectal carcinoma

目的:研究趋化因子受体CXCR4在结直肠癌组织中的表达,探讨其与淋巴结、肝转移及预后的关系。方法:应用免疫组化方法(SP法)检测20例正常结直肠黏膜组织、64例结直肠癌组织、34例区域淋巴结转移癌组织以及18例肝转移组织CXCR4表达情况。同时应用逆转录聚合酶链反应(RT-PCR)检测其mRNA在4株大肠癌细胞株中的表达。结果:正常结直肠黏膜、结直肠癌、区域淋巴结转移癌以及肝转移组织中CXCR4阳性率分别为15.0%、51.6%、72.2%和73.5%;转移组织阳性表达率明显高于原发肿瘤。CX-CR4 mRNA高表达于2株人大肠癌细胞株。结直肠癌组织CXCR4阳性表达率与Duke临床分期、淋巴结转移和肝转移密切相关,P<0.05,而与患者年龄、性别、肿瘤所在部位及病理分化程度无关。CXCR4阳性表达组3年生存率明显低于阴性表达组,P<0.05。结论:CXCR4阳性表达与结肠直肠癌的淋巴结、肝转移有关,有助于预后判断。

OBJECTIVE： To investigate the expression of che mokine receptor CXCR4 in colorectal carcinoma and its correlation with the clinicopathologic characteristics and prognosis. METHODS： The expression of chemokine receptor CXCR4 protein in colorectal carcinoma tissues obtained from 64 patients who underwent radical resection, 20 normal colorectal tissues, 34 local lymphatic metastais carcinomar tissues and 18 hepatic metastasis tissues was detected by immunohistochemistry. Simultaneously, the expression of chemokine receptor CXCR4 mRNA in 4 colorectal carcinoma cell lines was examined by reverse transcription polymerase chain reaction. RESULTS： Two of 4 colorectal carcinoma cell lines expressed CXCR4 mRNA. The positive rates of CXCR4 in normal colorectal, colorectal carcinoma tissues, cocal lymphatic metastasis carcinomar tissues, and hepatic metastasis tissues were 15.0%,51.6%, 72.2% and 73.5%, which were correlated with lymph node metastasis, clinical staging and liver metastasis （P〈0.05）, but not with the sex and age of patients, tumor location, T staging, and pathological types. Three-year survival rate in CXCR4 positive group was significantly lower than that in CXCR4 negative group. CONCLUSION： Positive expression of CXCR4 by colorectal cancer cells is significantly associated with lymphatic and he patic metastasis in patients with colorectal cancer, and may be used as a potential predictive factor for the prognosis of colorectal cancer.