分光光度法测定血清中的葡萄糖

Spectrophotometric determination of glucose in serum

在盐酸介质中，利用葡萄糖与亚硫酸钠及对氨基偶氯苯反应生成红色化合物的性质，建立了分光光度法测定葡萄糖含量的新方法。最大吸收波长为525um，线性范围0．02～0．2mg/25mL，检出限为0．sμg／mL，方法的相对标准们差为1－01％。将本方法用于血清中葡萄糖测定，结果令人满意。

Based on the reaction of glucose with sodium sulfide and p-aminozaobenzenein in hydrochloric acid forming a purplish-red solution, a spectrophotometric method for determination of glucose in serum was established. The maximum ab-sorbance was at 525nm. The rectilinear rang was O-O2 ~ O.2rngQ5rnL for glucose with the lower detection limit of0. 5ug/mL and RSD was 1.01 %. The method has been used for the determination of glucose in human serum with satis-for.