脑心通胶囊对大鼠脑缺血再灌注损伤的保护作用

Protective Effects of Naoxintong Capsule on Cerebral Ischemia-reperfusion Injury in Rats

目的探讨脑心通胶囊对大鼠脑缺血再灌注损伤的保护作用。方法45只SD大鼠随机分为5组:假手术组(5只)、缺血再灌注组(10只)、脑心通胶囊小剂量组(10只)、脑心通胶囊大剂量组(10只)、辅酶Q10组(10只)。采用双侧颈总动脉结扎法制作不完全性脑缺血再灌注模型,分别观察脑缺血30min再灌注30min及缺血30min再灌注60min脑组织含水量和ATP酶活性。结果缺血再灌注组与假手术组比较,Na+-K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶活性降低,脑含水量增加,差异有非常显著性意义(P<0.01);脑心通胶囊组与缺血再灌注组比较,Na+-K+-ATP酶、Ca2+-ATP酶和Mg2+-ATP酶活性增加,脑含水量降低,且差异有非常显著性意义(P<0.01)。结论脑心通胶囊对脑缺血再灌注损伤有保护作用。

Objective To investigate the protective effects of Naoxintong capsule on cerebral ischemia-reperfusion injury in rats. Methods 45 SD rats were randomly divided into 5 groups： sham operation group （n= 5）, ischemia-reperfusion group （n= 10）, small dose of Naoxintong capsule group （n= 10）, large dose of Naoxintong capsule group （n= 10）, CoQ-10 group （n= 10）. The the cerebral iscbemia-reperfusion model was established by occluding bilateral carotid arteries of animal. The moisture content and activity of ATPase in brain tissue of model rat were observed respectively at ischemia 30 min plus reperfusion 30 min and ischemia 30 min plus reperfusion 60 min. Results The activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase and Mg^2＋ -ATPase decreased （P 〈0.01）, contents of water in brain tissues increased （P 〈0.01） in the ischemia-reperfusion group, compared with the sham operation group. The activi ties of Na^＋-K^＋-ATPase, Ca^2＋ ATPase and Mg^2＋-ATPase increased （ P d0.01）, contents of water in brain tissues decreased （ P 〈0.01） in the Naoxintong capsule group, compared with the ischemia-reperfusion group. Conclusion Naoxintong capsule can protect the brain tissue from ischemia reperfusion injury.