清感颗粒提取及醇沉工艺研究

Water extraction and alcohol precipition of Qing gan granule

目的:优选清感颗粒的提取工艺及醇沉工艺.方法:采取正交试验,以挥发油含量、浸膏得率和绿原酸含量为考察指标,对清感颗粒提取工艺及醇沉工艺进行优选.结果:最佳提取工艺为:加水提取3次,每次加8倍量水,提取2h,首次浸泡60min.最佳醇沉浓度为70%.结论:该工艺可以提高清感颗粒有效成分提取率和纯度.

AIM： To optimize the water extracting technology and alcohol precipitation conditions of Qinggan granule. METHODS： The extraction procession was studied by orthogonal design with volatile oil extract yield and chlorogenic acid as the detective indices. Effects of 4 factors and 3 levels for each factor extraction rate were analyzed. RESULTS： The chlorogenic acid extraction rate reached over 58% under the optimum extraction conditions： 8 folds of water, 3 times, decoction 2 h once Soaking 60 min before the first time, precipitation with 70% alcohol. CONCLUSION： The optimum water-decocting condition is repeatable, and ensures stable chlorogenic acidas extraction rate. It could be used in the future large-scaled preparation of Qinggan granule.