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甲状腺乳头状癌RASSF1A启动子异常甲基化的检测 被引量：1

The Methylation of RASSF1A Gene in Papillary Thyroid Carcinoma
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摘要 为了研究甲状腺乳头状癌组织中RASSF1A基因启动子区甲基化状态,探讨其甲基化与临床参数之间的关系,运用甲基化特异性PCR(MSP)技术,对51例甲状腺乳头状癌组织及10例正常甲状腺组织中RASSF1A基因的甲基化状况进行分析。结果表明:甲状腺乳头状癌组织中RASSF1A基因甲基化率为68.6%(35/51),而正常甲状腺组织中未检测到该基因的甲基化,两者之间的差异有统计学意义(P<0.05);RASSF1A基因甲基化与患者年龄、性别无相关性与肿瘤的浸润和转移有相关性,差异有统计学意义(P<0.05)。因此,RASSF1A基因甲基化是甲状腺乳头状癌组织中常见的分子生物学事件,在腺癌的发生发展过程中发挥重要作用。 To observe the methylation status of RASSF1A gene in papillary thyroid carcinoma and its correlation with clinical paraments. Methods： Methylation-specific PCR （MSP） was used to analyze the methylation status of RASSF1A gene in 51 papillary thyroid carcinomas and 10 normal thyroid tissues. Results： Hypermethylation of RASSF1A gene was observed in 68.6% （35/51） of FFC （pap illary thyroid carcinoma）. None of the normal thyroid tissues was methylated, compared to the carcinoma, P 〈 0.05. There was no evidence to demonstrate the relationship between the methylation of RASSF1A gene and the patients＇ age and gender （P 〉 0.05）. The methylation of RASSFIA gene was positively related with metastasis （ P 〈 O. 05）. Conclusion： The methylation of RASSF1A gene is one of the important molecular biological events and is related to metastasis, which may be involved in thyroid tumorigenesis.
作者 王新华 张国昌 赵瑾 农卫霞
机构地区 石河子大学医学院
出处 《石河子大学学报（自然科学版）》 CAS 2008年第5期532-534,共3页 Journal of Shihezi University(Natural Science)
基金 新疆兵团专项基金项目(NKBo2sDXNK34)
关键词 乳头状癌 基因 RASSF1A 甲基化 papillary thyroid carcinoma gene RASSF1A methylation
分类号 R363.21 [医药卫生—病理学]
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参考文献10

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同被引文献8

  • 1Dammann R,Li C,Yoon J H,et al.Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3[J].Nat Genet,2000,25:315-319.
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  • 4Schagdarsurengin U,Gimm O,Hoang-Vu C,et al.Frequent epigenetic silencing of the CpG island promoter of RASSF1A in thyroid carcinoma[J].Cancer Res,2002,62:3698-3701.
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  • 7Xing M,Cohen Y,Mambo E,et al.Early occurrence of RASSF1A hypermethylation and its mutual exclusion with BRAF mutation in thyroid tumorigenesis[J].Cancer Res,2004,64:1664-1668.
  • 8唐建东,欧阳军,栗夏莲,杜潇潇,邱新光,张全武.甲状腺乳头状癌组织中RASSF1A基因启动子甲基化与临床病理学的关系[J].天津医药,2007,35(12):887-889. 被引量：3

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石河子大学学报（自然科学版）
2008年 第5期

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