川芎嗪对急性百草枯中毒大鼠肝组织NO和iNOS的影响

Effects of ligustrazine on NO and iNOS in rats with acute hepatic injury induced by Paraquat

目的探讨川芎嗪对急性百草枯中毒大鼠肝组织一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的影响及作用机制。方法将50只SD大鼠随机分成5组,空白对照组、阴性对照组、阳性对照组、川芎嗪低剂量组和川芎嗪高剂量组。观察大体标本、组织病理,测定血清中ALT、AST、丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平,同时测定肝组织NO含量和iNOS活性。结果与阴性对照组比较,阳性对照组及川芎嗪低剂量组血清ALT、AST、MDA及肝组织NO浓度、iNOS活性均降低,血清SOD、GSH-Px均升高(P<0.01,<0.05);而川芎嗪高剂量组血清ALT、AST、MDA及肝组织NO浓度、iNOS活性无统计学意义;肝组织病理损伤也显著减轻。结论川芎嗪对百草枯诱导的肝损伤具有保护作用,其作用可能是通过抑制NO水平和iNOS活性而实现的。

Objective To investigate the effects of ligustrazine on nitric oxide （NO） and inducible nitric oxide synthase （iNOS） in rats with acute hepatic injury induced by Paraquat. Methods 50 Sprague- Dawley （SD） rats were randomly divided into five experimental groups： blank group, negative control group, positive control group, ligustrazine lowdose group（ LDG）, ligustrazine high-dose group（HDG）. Macroscopic and histopathological examinations were performed. In the mean time, the level of ALT, AST, MDA, SOD and GSH-Px in pIasma were detected, and the activities of hepar tissue homogenate NO and iNOS were measured. Results Compared with that in negative control group, ALT, AST, MDA, SOD and GSH-Px in positive control and ligustrazine low-dose group had statistical significance （P 〈 0. 05, 〈 0.01 ）, and biological indexes of hepatic injury and the contents of NO and iNOS were significantly decreased （P 〈 0.05, 〈0.01 ）. Hemorrhage of liver of ligustrazine low-dose group were mitigated. But that in ligustrazine high-dose group dont have statistically significant. Conclusions Ligustrazine has protective effect on hepatic injury induced PQ and their therapeutic mechanism is possibly via inhibiting the activities of NO and iNOS.