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GRIM-19在卵巢癌细胞株中的高表达及其意义 被引量：7

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摘要 将高表达GRIM-19的重组质粒经脂质体转染到卵巢癌细胞株(SK-OV-3)中,构建稳定高表达GRIM-19的SK-OV-3/GRIM-19细胞株,并设SK-OV-3/Control对照细胞株。通过逆转录聚合酶链反应和免疫印迹法检测GRIM-19及其下游基因信号转导和转录激活子3(STAT3)的表达,运用四甲基偶氮唑蓝法检测细胞增殖情况。结果成功建立了高表达GRIM-19的SK-OV-3/GRIM-19细胞株,并发现STAT3的表达明显下调;MTT检测结果显示,SK-OV-3/GRIM-19细胞株增殖较SK-OV-3/Control细胞株明显下调(P<0.05)。提示GRIM-19的高表达可以抑制卵巢癌细胞株的增殖,并且下调STAT3的表达。
作者 李敏 周颖 彭程 冯定庆 蒋丽莎 凌斌 肖卫华
机构地区 安徽医科大学附属省立医院妇产科分子实验室安徽省分子医学重点实验室 中国科技大学生命科学院
出处 《山东医药》 CAS 北大核心 2009年第1期78-80,共3页 Shandong Medical Journal
关键词 GRIM-19 信号转录和转录激活子3 SK-OV-3/GRIM-19
分类号 R737.31 [医药卫生—肿瘤]
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参考文献6

  • 1Seo T, Lee D, Shim YS, et al. Viral interferon regulatory factor I of Kaposi's sarcoma-associated herpesvirus interacts with a cell death regulator, GRIM-19, and inhibits interferon/retinoic acid-induced cell death[ J]. J Virol, 2002,76( 17 ) :8797-8807.
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二级参考文献10

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共引文献8

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二级引证文献32

山东医药
2009年 第1期

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