Tyr^3-octreotide 的 ^(131)I 标记条件研究及其在小鼠体内的生物分布

THE LABELLING CONDITIONS STUDIES OF TYR 3 OCTREOTIDE WITH 131 I AND ITS BIODISTRIBUTION IN MOUSE

进行了１３１Ｉ标记Ｔｙｒ３－ｏｃｔｒｅｏｔｉｄｅ最佳标记条件的研究，包括反应量、反应时间、反应体积等对标记率的影响。ＨＰＬＣ分析最高标记率可达８２％；用ＳＥＰ－ＰＡＫ柱处理后，ＨＰＬＣ分析放化纯度＞９９％。对纯化后标记物在小鼠体内的生物分布也进行了研究，结果表明：血液清除快，标记物通过肝胆排泄；标记物在肿瘤内有一定浓聚，在注入后３ｈ内显像效果较好。

The labelling conditions of Tyr 3 octreotide with 131 I by Ch T are studied. The labelled yield is 82% by HPLC. After purification using the Sep Pak C 18 reversed phase cartridge, the radiochemical purity is 99% by HPLC. The results of in vivo biodistribution in mouse show 131 I Tyr 3 octreotide is repidly cleared from the circulation mainly by the liver. The metabolism of 131 I Tyr 3 octreotide in tumor bearing mouse is slower than that in control mouse. At three hours after intravenous injection, good localization in tumors is observed.