稳恒磁场对小鼠骨髓细胞微核和血象及脑组织脂质过氧化的影响

Effect of Static Magnetic Fields on Polychromatic Erythrocyte Micronucleus, Blood Cells and Brain Tissue Lipid Peroxidation of Mouse

目的研究稳恒磁场对小鼠骨髓嗜多染红细胞(PCE)微核率、血象和脂质过氧化的影响。方法将40只清洁级昆明小鼠随机分为阴性对照组,阳性对照组(CP:145 mg/kg)、稳恒磁场暴露组(20,40,50,60,80,100 mT),暴露组分别暴露于不同强度稳恒磁场,每天2次,每次2 h,暴露15 d后检测骨髓嗜多染红细胞微核率并进行血象分析,同时测定脑组织超氧化歧化酶(SOD)活力和丙二醛(MDA)含量。结果稳恒磁场暴露组(50,60,80,100 mT)小鼠微核率较阴性对照组明显增高(P<0.05),而红细胞计数和白细胞分类有明显变化。当稳恒磁场为60、80、100 mT时,小鼠脑组织中的SOD活力低于对照组(P<0.05),小鼠脑组织中的MDA的含量高于对照组(P<0.05)。结论当小鼠受到稳恒磁场(50、60、80、100mT)作用时,骨髓嗜多染红细胞微核率增加,红细胞计数和白细胞分类及脑组织中脂质过氧化有明显改变。

Objective To study the effect of static magnetic fields on the micronucleus in polychromatic erythrocytes （PCE） in the bone marrow and and the lipid peroxidation in the brain tissue in mice. Methods Fourty Kunming mice were randomly divided in to the negative control group, positive control group（cyclophosphamide： 145 mg/kg） and the static magnetic fields （20, 40, 50, 60, 80 and 100 roT） exposure group, exposed twice a day, for two hours each time. After 15 days ,the micronuclei rate of the bone marrow, blood cells and the activity of superoxide dismutase （SOD）, the protein malonaldehyde （MDA） were determined. Results Compared with the control, the micronuclei rate in 50, 60, 80 and 100 mT exposure group increased significantly, the amount of leukocyte significantly decreased, the activity of SOD in the brain tissue in 60, 80 and 100 mT exposure group decreased and the content of MDA increased significantly. Conclusion The present experiment demonstrats that static magnetic fields exposure at doses of 50, 60, 80 and 100 mT may induce the micronuclei rate increase, the amount of leukocyte decrease, the activity of SOD in the brain tissue decrease and the content of MDA increase.