农产品中有机磷农药的小麦酯酶抑制测定法的评价

Wheat Esterase Inhibition Method for Determination of Organic Phosphorus Pesticide in Agricultural Products

目的评价小麦酯酶抑制法检测农产品中有机磷农药残留的应用效果。方法对小麦酯酶抑制法测定韭菜中有机磷农药残留进行了质量评价和主成分分析。检测了5种农产品中有机磷农药残留量,并以气相色谱法进行了确证试验。结果不同有机磷农药检出下限的范围为0.05～0.52 mg/kg,标准差为0.26～3.09 mg/kg,RSD为2.9%～4.1%,平均回收率为74.5%～92.1%。有机磷农药对酶的抑制程度和精密度是影响测定质量的主要因素。结论小麦酯酶抑制法可以满足农产品中有机磷农药残留半定量分析的要求。

Objective To estimate the application of the wheat esterase inhibition method for the determination of organic phosphorus pesticide residues in agricultural products. Methods The wheat esterase inhibition method was used for the determination of organic phosphorus pesticide residues in chive, the quality evaluation and principal component analysis were conducted. Organic phosphorus pesticide residues in five agricultural products were detected by this method, and compared with the confirmatory test with GC. Results The detection limit, standard deviation, relative standard deviation and average recovery of this method for various organic phosphorus pesticides were 0.05-0.52 mg/kg, 0.26-3.09, 2.9%-4.1% and 74.5%-92.1%, respectively. The inhibitory degree of pesticide and precision was the primary factor for influencing the result of quality evaluation. Conclusion The wheat esterase inhibition method is reasonable and is applicable for the semi-quantitative analysis of organic phosphorus pesticide residues in the agricultural products