柱花草种质遗传多样性的ISSR分析

An analysis by ISSR of genetic diversities in Stylosanthes germplasms

应用ISSR 标记,对48份柱花草种质的遗传多样性进行研究。从68 个ISSR 引物中筛选出14 个多态性明显。反应稳定的引物,共扩增出156条谱带,平均每个引物能扩增出11.1条带,多态性条带比率达99.36%。材料间遗传相似系数为0.445-0.975,POPGENE 结果分析表明,平均Shannon信息指数（I）为0.3553,平均Nei＇s基因多样性（H）为0.2279,每位点平均有效等位基因数（NE）为1.3887。利用聚类分析和主成分分析表明,48份供试材料可聚为5类：有钩柱花草类。头状柱花草类。圭亚那柱花草类。西卡柱花草类和灌木柱花草类,其中,圭亚那柱花草类种内材料遗传变异较大。

Forty-eight Stylosanthes germplasms were studied for an analysis of genetic diversity by ISSR. Fourteen effective primers selected from 68 ISSR primers were used for PCR, and 155 of the 156 DNA fragments amplified, showed polymorphisms. The average binds from each primer was 11.1 and the average percentage of polymorphic bands was 99.36 %. The coefficient of genetic conformability among the germplasms was 0.445-0. 975. The results from POPGENE analysis indicated that the average information index （I） of Shannon was 0. 355 3, gene diversity （H） of Nei was 0. 227 9, and effective number of alleles （NE） was 1. 388 7. Based on analyses of cluster and principal components, the 48 Stylosanthes germplasms used in this study could be divided into 5 groups： S. hamata, S. capitata, S. scabra, S. seabrana and S. guianensis, in which the genetic differentiation of S. guianensis group was the largest.