猪血红蛋白抗氧化活性肽的分离及脱色技术研究

Research on Isolation and Decolourization Technology of Antioxidative Porcine Hemoglobin Peptides

为充分挖掘血液蛋白质资源及开发具有保健功能的血液深加工生物制品,本实验以猪血为原料,以猪血红蛋白为研究对象,采用胃蛋白酶在pH1.6,酶底比1.6%,温度40.4℃条件下对猪血红蛋白酶解60min,后采用中空纤维超滤设备(截留分子量分别为10KDa,5KDa和3KDa)对酶解产物进行分离和脱色,获得4组区分物(PHH-Ⅰ∶MWCO>10KDa;PHH-Ⅱ∶10KDa>MWCO>5KDa;PHH-Ⅲ∶5KDa>MWCO>3KDa;PHH-Ⅳ∶MWCO<3KDa)。结果显示,各区分物随分子量的降低其色泽逐渐变浅,区分物PHH-Ⅳ具有最高抗氧化活性,且其接近无色,透明、清澈,感官质量得到了很大提升,在功能性食品、药物及化妆品工业领域极具应用潜力。

In order to fully excavate blood protein resources and develop the high added value blood protein deep processed product with health care function, this experiment adopted porcine blood as raw materials and porcine hemoglobin as research object, and this study adopted pepsin to hydrolyze porcine hemoglobin under pill.6, enzyme/substrate=l.6%, temperature 40.4℃, time 60min. The hydrolysate was isolated and decolourized by hollow fiber ultrafiltration apparatus （molecular weight cutoffs： 10KDa, 5KDa and 3KDa, respectively） into 4 fractions （PHH-Ⅰ： MWCO〉10KDa; PHH- Ⅱ： 10KDa〉MWCO〉5KDa; PHH-Ⅲ： 5 KDa 〉 MWCO 〉 3KDa; PHH-Ⅳ： MWCO〈3KDa）. The results showed that the color of fraction was gradually from dark to light with the molecular weight cutoff decreased. Fraction PHH- Ⅳ showed the strongest antioxidant activity. Its color was near colorless, transparent and limpidity and the sense quality was enhanced greatly. Fraction PHH- Ⅳ has enormous applied potentiality in the fields of function food, medicine and cosmetic industries.