抗氧化剂对溃疡性结肠炎患者的治疗作用及其机制

Therapeutic effects of antioxidant on inflammatory injury and relevant mechanism in patients with ulcerative colitis

目的探讨抗氧化剂还原型谷胱甘肽,维生素C、E对溃疡性结肠炎(UC)患者的治疗作用及其作用机制。方法检测42例溃疡性结肠炎患者和35例健康对照者的血SOD,MDA,LPO,NO,GSH-Px的水平变化,同时检测结肠炎过氧化物酶(MPO)活性,评价UC患者病情活动指数(CAI)与内镜分级。结果UC患者组血MDA、LPO和NO水平及结肠组织MPO活性显著高于正常对照组,血SOD和GSH-Px活性显著低于正常对照组(P<0.01),抗氧化剂还原型谷胱甘肽,维生素C、E治疗后可明显降低UC患者血MDA、LPO和NO水平,增加SOD和GSH-Px活性,同时CAI评分与内镜分级亦显著得到改善(P<0.01)。结论抗氧化剂还原型谷胱甘肽,维生素C、E能显著抑制UC患者氧化损伤,减轻结肠炎症损伤,具有临床治疗价值。

[Objective] To investigate the therapeutic effects of antioxidant GSH, Vitamin C and E on inflammatory injury and relevant mechanism in patients with ulcerative colitis. [Methods] Forty-two patients with UC and thirty-five controls were enrolled. Malondiadchyde （MDA）, Lipid superoxide （LPO）, Nitric oxide （NO）, activity of superoxide dismutase （SOD） and Glutathione peroxidase （GSH-Px） in blood and activity of myeloperoxidase （MPO） in colon were detected by corresponding kits, respectively. At the same time, CAI grade and endoscope grade were evaluated. [Results] Levels of MDA, LPO, NO in blood and activity of MPO in colon of patients with UC were significantly increased compared with healthy controls while activity of SOD and GSH-Px in blood were significandy decreased （P 〈0.01）.After antioxidant treatment, levels of MDA, LPO, NO and activity of MPO were remarkably reduced while activity of SOD and GSH-Px were significantly increased in patients with UC （P 〈0.01）. [Conclusion] Antioxidant reduces colonic inflammatory injury and ameliorate the colitis in patients with UC, Antioxidant can be considered as a novel therapeutic ahematives for the treatment of IBD.