墓头回提取液对小鼠移植瘤U14瘤体内微血管密度VEGF和bFGF表达的影响

Effect of the extracting solution from mutouhui on the microvessel-density and VEGF/bFGF expression in the U14 replanted tumor tissues of the mice that had cervical carcinoma

目的探讨墓头回提取液对小鼠宫颈癌U14移植瘤瘤体组织微血管密度、VEGF、bFGF表达的影响。方法将SPF级雌性KM小鼠随机分成墓头回提取液高、中、低剂量组、顺铂组及模型组，以观察墓头回提取液对U14实体瘤肿瘤组织微血管密度、血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）表达的影响。结果墓头回提取液高、中剂量组和顺铂组的宫颈癌U14移植瘤瘤体内微血管密度均明显低于模型组；墓头回提取液高、中剂量组和顺铂组均可降低U14移植瘤瘤体内VEGF的表达；墓头回提取液高剂量组和顺铂组均可降低U14移植瘤瘤体内bFGF的表达。结论墓头回提取液能明显抑制U14移植瘤瘤体血管形成，并能降低VEGF、bFGF的表达，提示墓头回提取液有抗肿瘤作用。

Objective To explore the effect of the extracting solution from mutouhui on the microvesseldensity and VEGF/bFGF expression in the U14 replanted tumor tissues of the mice that had cervical carcinoma. Methods The SPF female mice were divided into high dose/middle dose/light dose of the extracting solution group, cisplatin group and model group randomly. Then the effect of the extracting solution from mutouhui on the microvessel-density and VEGF/bFGF expression in the U14 replanted tumor tissues of the mice that had cervical carcinoma were observed. Results The microvessel-density in high dose/middle dose of extracting solution group and eisplatin group was lower than that in model group; VEGF expression in high dose/middle dose of extracting solution group and eisplatin group was lower than that in model group; bFGF expression in high dose/ middle dose of extracting solution group and cisplatin group was lower than that in model group. Conclusion The extracting solution from mutouhui is against the formation of the microvessel-density in U14 transplanted tumor, and it also can reduce the VEGF/bFGF expression, all these show that extracting solution from mutouhui has the effect of anti-tumor.