α-actinin-4在糖尿病大鼠的表达及贝那普利对其的干预作用

The expression of α-actinin-4 in diabetic rats and the effect of benzapril on α-actinin-4 expression

目的探讨糖尿病肾病发展过程中α-actinin-4的表达变化及贝那普利对其的干预作用。方法建立STZ诱导的糖尿病大鼠模型，贝那普利对其干预，应用免疫组化及RT-PCR方法观察在糖尿病肾病发展过程中α-actinin-4的蛋白及mRNA表达变化。结果8周时，糖尿病组染色强度较正常组明显增高（P〈0．01），12周时变化更为明显（P〈0．01）。α-actinin-4的mRNA的表达水平在各时间点与组化结果相同。贝那普利干预组8周时α-actinin-4表达较糖尿病组明显降低（P〈0．05），而在12周时其表达仍较糖尿病组低，但无统计学意义。结论α-actinin-4在糖尿病肾病发展过程中表达增高，贝那普利能下调其表达。

Objective To investigate the change of α-actinin-4 expression and the effect of benzapril on α- actinin-4 expression. Methods STZ-induced diabetic rats were established, benzapril was given to it, immunohistochemical and RT-PCR were employed to study the change of α-actinin-4 protein and mRNA. Results At week 8, compared with normal group,the expression of α-actinin-4 protein in diabetic group increased dramatically （ P 〈 0.01 ） ; At week 12, the expression still raised （ P 〈 0.01 ）. The expression of α-actinin-4 mRNA was similar to immunohistochemical staining. At week 8 , the expression of α-aetinin-4 protein in benzapril group decreased when compared with diabetic group（P 〈 0.05）; At week 12, it can also decreased, but had no statistical significance. Conclusion The expression of α-actinin-4 increased in diabetic nephropathy and benzapril can normalize it.