盐胁迫条件下盐藻的生长及特异表达蛋白的研究

Growth and Salt-induced Proteins in Alga Dunaliella salina at Different Salinities

采用不同NaCl浓度培养盐藻,通过分光光度法记录其生长状态。试验结果表明,在NaCl浓度为1.0～2.0mol/L时,盐藻生长状态良好;NaCl浓度为3.0～4.0mol/L时盐藻生长缓慢;盐藻经不同NaCl浓度培养0.5、5、23h后抽提蛋白,所获得的粗提蛋白经SDS-聚丙烯酰胺凝胶电泳分离,观察不同NaCl浓度培养液中生长的盐藻可溶性蛋白质SDS-PAGE图谱,发现在低NaCl浓度(1.0～2.0mol/L)下培养时23、51kD蛋白略有变化但变化不大;在高NaCl浓度(3.0～4.0mol/L)下胁迫5、23h时23kD蛋白显著增加;高NaCl胁迫23h时51kD蛋白含量下降明显。试验结果证明51、23kD蛋白可能与盐藻耐盐机制有关。

The growth of green alga Dunaliella salina was determined at different salinities by a spectrophotometric method. The alga was found to have good growth at a salinity of 1.0-2.0 mol/L and to have slow growth at high salinity （3.0-4.0 mol/L）. Crude protein was extracted from the cells 0.5 h, 5 h and 23 h after culture and then was subject to SDS-PAGE at different salinities. The 51 kD and the 23 kD protein showed slight changes at low salinities. At high salinities, however, the protein 23 kD level increased significantly 5 h, and 23 h after culture, but the protein 51 kD level decreased 23 h after culture through soluble protein profiles, indicating that the change in expression of proteins at different salinities was involved in mechanisms of salt-tolerance in alga Dunaliella salina.