多发性肌炎和皮肌炎外周血CD45RA^＋及CD45RO^＋细胞亚群的变化

Changes of CD45RA^＋ and CD45RO^＋ cell subsets in peripheral blood in polymyositis/dermatomyositis

目的：研究多发性肌炎（PM）和皮肌炎（DM）外周血单一核细胞（PBMCs）中CD45RA^＋、CD45RO^＋细胞亚群的变化。方法：应用双色荧光标记抗体、流式细胞仪对PM和DM外周血CD45RA^＋、CD45RO^＋细胞亚群进行了检测。结果：（1）PM和DM的PBMCs中CD45RA^＋细胞显著低于对照组，CD45RO^＋细胞和CD45RO^＋／RA^＋比值均显著高于对照组，PM的CD45RA^＋细胞显著低于DM（均P〈0．05）。（2）在PM的CD4^＋亚群中，CD45RA^＋细胞显著低于对照组和DM，CD45RO^＋细胞和CD45RO^＋／RA^＋比值显著高于对照组（均P〈0．05）。（3）在DM的CD4^＋亚群中，CD45RA^＋细胞与对照组比较无显著性差异（P〉0．05），CD45RO^＋细胞和CD45RO^＋／RA^＋比值显著高于对照组（均P〈0．05）。（4）在CD8^＋亚群中，PM和DM的CD45RA^＋、CD45RO^＋细胞与对照组比较，均无显著性差异（均P〉0．05），但CIM5RO^＋／RA^＋比值均显著高于对照组（均P〈0．05）。结论：在PM和DM自身免疫发病过程中PBMCs和CD4^＋、CD8^＋亚群中的CD45RO^＋、CD45RA^＋细胞可能起重要作用，PM和DM在免疫学方面存在一定的异质性。

Objective： To investigate the changes of CIMSRA^＋/CIMSRO^＋ cell subsets in peripheral blood mono- nuclear cells （PBMCs） in polymyositis （ PM ）/dermatomyositis （ DM ）. Methods： The changes of CD45RA^＋ and CD45RO^＋ cell subsets in peripheral blood of PM/DM were detected by two color flourescence labeled antibody and flow cytometry. Results： （1） The CD45RA^＋ cells in PBMCs in PM and DM were decreased significantly than that in control group and the CD45RO^＋ cells and the ratio of CD45RO^＋/CD45RA^＋ were both significantly higher as compared to control group. The number of CD45RA^＋ cells were significantly lower in PM than in DM （ P 〈 0.05）. （2） In PM, the number of CD45RO^＋ cells was significantly lower than in control group and in DM. The number of CD45RO^＋ cells and the ratio of CD45RA^＋/CD45RAO^＋ were both significantly higher than that in control group （ P 〈 0.05）. （3） In DM, the CD45RA^＋ cells was not significantly defferent from that in control group （ P 〉 0.05）, and the number of CD45RO^＋ cells and the ratio of CD45RO^＋/CD45RA^＋ were significantly higher than that in con- trol group （ P 〈 0.05）. （4） In CD8^＋ subset, the CD45RA^＋ cells and CD45RO^＋ cells in PM and DM were not significantly defferent from that in control group （ P 〉 0.05）, but the ratio of CD45RO^＋/CD45RA^＋ in PM and DM was significantly higher than that in control group （P〈0.05）. Conclusion： The rising of CD45RO^＋ cells in PBMCs, and the CD45RO^＋/CD45RA^＋ cells in CD4^＋ and CD8^＋ subsets may play an important role in the pathogenesis in PM and DM, which may present heterogeneity in immunology.