德国牧羊犬父母代与子代嗅觉受体基因序列分析与比较

Sequence Analysis and Comparison on Olfactory Receptor Genes of German Shepherd Dog between Parents and the Filial Generation

目的了解德国牧羊犬父本和母本以及他们的子代之间嗅觉受体基因遗传相关规律。方法以常规PCR技术扩增德国牧羊犬父母代与子代嗅觉受体基因，并进行相关基因的序列分析、比较。结果与母本嗅觉受体基因相比，子代和父本之间的嗅觉受体基因差异变化较大。子代和父母本之间的碱基突变主要有T→C、G→C、A→G、CVT、G→T、T→A和G→A类型突变。有些突变导致氨基酸改变。结论本文结果对CfOLF基因的多态性分析及对品种内的遗传规律研究有非常重要的意义。

Objective To investigate the heredity the of olfactory receptor genes between the parents and the filial generation of German shepherd dog. Method Olfactory receptor genes of the parents and the filial generation of German shepherd dog were amplificated using the routine PCR method, and the related olfactory receptor genes were sequenced and analyzed. Result Olfactory receptor genes differed much more between the filial generation and the male parent than between the filial generation and the female parent. The T to C, G to C, A to G, C to T, G to T, T to A and G to A transitions were the main mutation type between parents and the filial generation. Some transitions resulting in the alteration in the protein sequence. Conclusion The results will be helpful to the research on the polymorphism and the heredity rule of canine olfactory receptor genes.