新西兰兔实验感染TTV的研究

Study on Experimental Infection of TTV in NEW Zeland Rabbits

目的探讨TTV对家兔感染的敏感性。方法对成年家兔进行了三代TTV感染实验，除第一代采用人TTV阳性血清外，第二，第三代分别采用感染的第一、第二代血清作为感染源，对12只兔（每代4只）进行了传代试验。结果第一、第二、第三代兔血清中都有不同程度生化和肝脏病理改变，血清中进行Nest-PCR检测全为阳性，4例兔肝组织原位杂交（Dig—TTV-DNA）试验，3例为阳性，另1例为阴性，对一份阳性标本进行部分核苷酸分析，与日本株（AB008394）和CH1、CX2同源性分别是96．7％、98．3％和99．7％。结论家兔能被TTV感染，是一种敏感动物，可以作为一种实验模型动物。

Objective To explore the sensitivy of rabbits to TTV infection. Methods Twelve rabbits were divided into three groups as three generations used fot infectious passag experiment of TTV. Rabbits of the first generation were infected with positive serum of TTV, the second generation were infected with serum from the first generation and those of the third generation with serum taken from the second generation. Result Various degrees of biochemical alteration in serum and pathological changes in liver were found inall experimental rabbits. By nest-PCR,detection on the serum of third generation showed positive. Three of the rabbits were positive with Dig-TTV DNA probe and other one negative. The nucleotide sequence analysis of the positive samples of strain（AB008394） CH1 and JX strain TTV, showed the homology 96.7%, 98.3%, and 99.7% respectively, Conclusion Rabbits can be infected with TTV, and are sensitive animals for establishment of an animal model.