摘要
目的研究刺五加多糖(ASPS)对氧化损伤的海马神经元OGG1mRNA表达的影响,为揭示ASPS具有抵抗氧化损伤提供有力的实验依据。方法运用海马神经细胞原代培养技术,采用H2O2诱导建立细胞氧化损伤模型。观察细胞形态学变化;MTT法测定细胞活性;化学比色法检测细胞匀浆液中过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力;RT-PCR法检测OGG1mRNA的表达。结果形态学观察显示,与模型组比较,ASPS组细胞损伤程度明显减轻;ASPS组细胞活性(0.46)比模型组细胞活性(0.36)高(P<0.01);SOD、GSH-Px活力ASPS组显著高于模型组;ASPS能够明显提高OGG1mRNA的表达。结论ASPS能够增强OGG1mRNA的表达,并对海马神经元氧化损伤有保护作用。
Objective To research the effects of acanthopanaeis sentieosi polysaceharides (ASPS) on expression of OGG1 mRNA in hippocampal neurons damaged by oxidative stress and provide experimental evidence for revealing the antioxidative stress injury effect of ASPS. Methods The oxidative stress injury cell model was induced by H2O2. The change of cell morphology was observed. The activity of hippoeampal neurons was deteeted with MTT method. The vitalities of superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px) were detected by chemical colorimetry. The expression of OGG1 mRNA was detected by RT-PCR. Results Compared with model group, the morphology damage of hippocampal neurons were slighter, and the activities of hippocampal neurons in ASPS group (0.46) were higher than those of model group (0. 36) (P 〈0.01 ). The vitalities of SOD and GSH-Px in ASPS group were higher than those in model group. ASPS obviously raised the expression of OGG1 mRNA. Conclusions ASPS can enhance the expression of OGG1 mRNA and protect the hip- pocampal neurons from oxidative stress injury.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第4期396-399,共4页
Chinese Journal of Gerontology
基金
湖北省科技攻关计划(2006AA412C08)
关键词
刺五加多糖
海马神经元
氧化应激损伤
OGG1
MRNA
Acanthopanaeis sentieosi polysaccharides
Hippoeampal neurons
Oxidative stress injury
OGGI mRNA