Bcl-2、Fas和Bax凋亡调控基因在限食和脑老化小鼠海马神经元中的表达

Expressions of apoptosis-regulatory Bcl-2,Fas and Bax gene in the hippocampal neurons of restricted diet and aging brain mice

目的通过细胞凋亡调控基因Bcl-2、Fas和Bax在限食及脑老化小鼠海马神经元中的表达,研究限食与脑老化的关系。方法3月龄ICR雄性小鼠40只,随机分为4组:脑老化模型组、脑老化模型限食组、限食对照组、对照组,每组10只。10w后进行为期7dMorris水迷宫实验,实验完毕后进行Bcl-2、Fas和Bax免疫组织化学染色。结果①Fas、Bax阳性神经元数目脑老化模型组显著高于脑老化模型限食组、限食对照组显著低于对照组;与此相反,Bcl-2阳性神经元数目在脑老化模型限食组显著高于脑老化模型组,限食对照组显著高于对照组。②Morris水迷宫实验测试:脑老化模型组逃避潜伏期显著大于其他各组;撤台后小鼠的记忆性搜台游泳路程结果各组小鼠间均无显著差异。结论限食可明显降低脑内凋亡相关基因Fas、Bax的表达,同时上调抗凋亡相关基因Bcl-2的表达,从而发挥抗脑细胞凋亡及防止脑老化性学习能力下降的作用。

Objective To investigate the expressions of apoptosis-regulatory Bcl-2, Fas and Bax gene in the hippocampal neurons of restricted diet and aging brain ,nice. Methods 40 3-month male ICR mice were randomly divided into aging brain, restricted diet and aging brain, restricted diet and control groups, 10 mice in each group. After 10 weeks, Morris water maze was tested and the expressions of Bel-2, Fas and Bax were measured by immunohistoehemical staining. Results （1）The expressions of Fas and Bax positive cells in aging brain group were higher than those in restricted diet and aging brain groups, and those in restricted diet group were lower than those in control group. On the contrary, the expression of Bcl-2 in restricted diet and aging brain groups was significantly higher than that in aging brain group, and also did between restricted group and control group. （2）The escape latency time in aging brain group was longer than that in other groups, the swimming distance in all groups was similar. Conclusions By up-regulation of the expression of Bcl-2 and down-regulation of the expressions of Fas and Bax, restricted diet can resist hippocampus neurons apoptosis and prevent the decline in learning ability caused by brain aging.