水中隐孢子虫的活性及传染性检测

Detection of Cryptosporidium Viability and Infectivity in Water

论文介绍了动物感染模型、体外脱囊技术、荧光活体染色、动物细胞培养技术和RT-PCR等检测水中隐孢子虫卵囊活性及传染性的技术,比较了各方法用于水样分析中的适用性、可靠性以及费用等,随着IMS、细胞分类法等纯化手段不断完善,PCR技术对研究水中隐孢子虫的检测以及活性、传染性分析将会有更广阔的发展空间,我国应致力于开展快速检测卵囊活性与传染性的研究。

Several detection methods of cryptosporidium viability and infectivity including animal infectivity models, in vitro excystation, fluorogenic dyes. mammalian cell culture and RT-PCR were introduced in this paper, The applicability, reliability and cost of these methods applied to water sample detection were compared. With the improvement of purification methods such as IMS and FACS, the PCR technology will have wider development space for the research of detection of cryptosporidium viability and infectivity in water, and research should be directed at developing rapid methods for the detection of viable and infective oocysts in China.