1,25-二羟维生素D3法建立高钙血症大鼠模型的研究

Construction of a Hypercalcemia Rat Model Induced by 1,25-dihydroxy-cholecalcifero

目的:以1,25-二羟维生素D3诱导建立高钙血症动物模型。方法:将40只Wistar大鼠随机分为4组,每组10只。分别将1, 25-二羟维生素D3以高中低(4μg/kg,2μg/kg,0.5μg/kg)三个剂量组连续灌喂Wistar大鼠2周,空白对照组灌喂2 mL生理盐水眼球丛静脉取血后测定大鼠血钙值及血液的生理生化指标。结果:以2μg/kg浓度的1,25-二羟维生素D3灌喂之后,大鼠血钙值显著上升(P<0.05),同时其它生理生化指标和对照组无明显差异。注射降血钙药物1.25 mg/kg帕米膦酸二钠(pamidronate)或200mIU/kg密钙息(Miacalcic)均能有效抑制该模型大鼠的血钙升高。结论:采用1,25-二羟维生素D3为诱导物是建立实验性高钙血症大鼠模型的一种可行方法。

Objective： To get the experimental rat model of hypercalcemia induced by 1, 25-dihydroxy -cholecalcifero. Methods： 40 Wistar rats were divided into four groups （n=10, each group）. Rats of the high dosage group （4μg/kg）, middle dosage group （2μg/kg） and low dosage group （0.5μg/kg） were respectively administrated with 1, 25-dihydroxy-cholecalcifero for two weeks. Each rat in the control group was administrated with 2 mLnormal saline. Physiological and biochemical indexes in the blood from ophthalmie venous plexus were measured. Results： The serum calcium concentration of the rats significantly increased in middle dosage group. At the same time, no significant change was found in the other detection indexes. Injecting 200 mIU/kg Miacalcie or 1.25 mg/kg pamidronate could decrease the serum calcium concentration. Conclusions： It is a feasible way to get hypercalcemic rat model with 1,25-dihydroxy-eholecal- cifero.