白屈菜红碱对宫颈癌细胞的抑制作用研究

Inhibition Effects of Chelerythrine on Hela Cells

目的:研究白屈菜红碱(chelerythrine,CHE)对宫颈癌细胞(Hela)的增殖抑制作用,为CHE在预防和治疗宫颈癌方面提供实验依据。方法:不同浓度CHE作用于体外培养的Hela细胞后,采用台盼蓝拒染法和噻唑蓝(MTT)法研究白屈菜红碱对宫颈癌(Hela)细胞增殖能力的影响,流式细胞仪(FCM)检测细胞周期变化。结果:细胞接种密度越小、药物作用时间越长、药物浓度越大, CHE对Hela细胞的增殖抑制率越大,过大的细胞接种密度以及过低的药物浓度均不会引起明显的细胞死亡;CHE作用后的Hela细胞周期分布有明显变化,G1期细胞减少,S期和G2/M期细胞明显增多。结论:CHE在体外能明显抑制Hela细胞生长,其机理可能与CHE阻滞细胞周期、诱导细胞凋亡有关。

Objective： To study the inhibitory effects of CHE on Hela cells in vitro, and provide the theory basis for prevention and treatment of cervical cancer. Methods： Hela cells were treated with various concentration of CHE for 24, 36, 48 and 60 hours. The cell proliferation was estimated by the MTT assay. Distribution and apoptosis of the cell cycle were examined by Flow cytometry. The morphological change of the cells was observed with optical-microscope directly. Results： The growth of Hela cells has been significantly inhibited by CHE. The inhibition rate increases with the decrease of cell inoculums density, the increase of treating time and the increase of drug concentration. No evident death phenomenon was observed with excessive inoculums density or too low drug concentration. The cell cycle was redistributed： the Gl-phase cell fraction was decreased, while the S-phase and G2/M-phase cell fraction were significantly increased after treated with CHE. Conclusions： CHE can inhibit the growth of Hela cells, and the mechanism may be related to its blockage of cell cycle and apoptosis induction.