苦草与铜绿微囊藻的相互化感作用

The mutual allelopathy of Vallisneria spiralis Linn.and Microcystis aeruginosa

在排除细菌作用和营养竞争的实验室条件下,对苦草和铜绿微囊藻进行混合培养和分开培养,探讨了苦草和铜绿微囊藻的相互化感作用.结果表明,苦草的存在对铜绿微囊藻的生长有明显的抑制;铜绿微囊藻对苦草生长的抑制,必须与藻对苦草的遮光作用相结合才能完成.藻的丙二醛(MDA)积累和藻叶绿素a含量降低表明,苦草释放的化感物质可能造成了铜绿微囊藻细胞内活性氧的增多,影响了正常的光合作用,导致藻细胞死亡.铜绿微囊藻则是通过减弱光照和减少苦草叶绿素a含量,导致苦草生物量减少.

Mutual allelopathy between Vallisneria spiralis Linn. and Microcystis aeruginosa was investigated through co-culture and separate culture, excluding the effects of bacteria and nutrient. Vallisneria spiralis Linn. could inhibit growth of M. aeruginosa, and M. aeruginosa could inhibit the growth of Vallisneria spiralis Lin only under shading conditions. In present study, accumulation of malondialdehyde （MDA） and decrease of chlorophyll a contents of M. aeruginosa were observed, indicating the excessive accumulation of reactive oxygen species and inhibition of photosynthesis might be the cause of the growth inhibition of alga. However, inhibition of Vallisneria spiralis Linn growth might due to decrease of chlorophyll a contents and M. aeruginosa shading.