Vc对体外培养的大鼠海马神经元生长发育的影响

EFFECT OF ASCORBIC ACID ON THE GROWTH AND DEVELOPMENT OF RAT HIPPOCAMPAL NEURONS IN CULTURE

用新生大鼠海马神经细胞进行体外分散培养，观察不同剂量Ｖｃ对神经细胞生长发育的影响。结果表明，１０－４ＭＶｃ组接种１６ｈ细胞的成活数明显高于对照（不加Ｖｃ），１０－５ＭＶｃ、１０－６ＭＶｃ组细胞的成活数与对照无显著差异，１０－３ＭＶｃ组细胞几乎全部死亡；１０－４ＭＶｃ使细胞的总蛋白量增加，细胞存活数细胞的突起长度和胞体面积均高于对照。提示１０－４ＭＶｃ对神经细胞生长发育有一定的促进作用。

The effects of Vc on the growth of hippocampal neurons from newborn rat were investigated by dissociated cell culture On the 16 Hr, the number of survival neurons in 10 -4 M Vc group was significantly higher than that of control group (no Vc in medium)(P<0.05).The number of survival neurons in 10 -5 M and 10 -6 M Vc groups was almost the same as control group, and the survival neurons in 10 -3 M Vc group were scanty. The number and the length of neuronsal processes as well as the area and maximum diameter, minimum diameter of neuronsoma in 10 -4 M Vc group were all increased markedly. the total contents of protein in 10 -4 M Vc group was enhanced significantly as compared with control. these results indicated that the suitable amount of Vc supplementation could improve neuron growth and development in vitro.