闲庭喷鼻粉剂对青霉素致痫大鼠作用的实验研究

Effect of Xianting nasal spray on rats with epilepsy induced by penicillin

目的观察闲庭喷鼻粉剂对青霉素致痫模型大鼠的作用效果,探讨经鼻给药法治疗癫痫的可行性。方法将40只Wistar大鼠随机分为空白组、模型组、实验低剂量组和实验高剂量组,每组10只。实验组经鼻腔给予临时配制的低、高剂量闲庭喷鼻粉剂混悬液,空白组和模型组则经鼻腔给予相应剂量的蒸馏水。10d后,模型组与实验组腹腔注射青霉素复制癫痫模型(空白组则腹腔注射等量注射用水),观察并比较4组大鼠的行为变化、癫痫发作潜伏期及发作程度。结果与模型组相比,闲庭喷鼻粉剂治疗组大鼠癫痫发作的潜伏期明显延长,癫痫发作程度明显降低。结论闲庭喷鼻粉剂对青霉素致痫模型大鼠有显著的治疗作用,通过经鼻给药的方式治疗癫痫是可行的。

Objective To observe the effect of Xianting nasal spray on epileptic rats induced by penicillin and analyze the feasibility of treating epilepsy by nasal administration. Methods 40 Wistar rats were divided into 4 groups randomly （ n = 10） ： normal control group, epileptic model group, low dose treated group and high dose treated group. The experimental groups were assigned to receive low dose or high dose suspension of Xianting nasal spray by nasal administration. The normal control group and the epileptic model group were given equal volume of distilled water. 10 d later, the epileptic model group and the experimental groups were injected with penicillin intraperitoneally, and the normal control group was treated with equal volume of aqua for injection. The behavior, latent period of epilepsy seizure and level of epilepsy attack among 4 groups were observed and compared. Results Compared with epileptic model group, Xianting nasal spray treated groups showed a prolonged latent period and diminished level of epileptic behavior. Conclusions Xianting nasal spray had a conspicuous anti-epilepsy effect on epileptic rats induced by penicillin. It is feasible to treat epilepsy by way of nasal administration.