全缘绿绒蒿药材质量标准研究

Quality Control of Meconopsis integrifplia

目的:建立全缘绿绒蒿药材中槲皮素的薄层色谱鉴别与含量测定方法。方法:采用TLC法对全缘绿绒蒿进行鉴别;采用HPLC法测定槲皮素含量,色谱条件为:色谱柱,Phenomenex LunaC18(2)(4.6mm×150mm,5μm);流动相,甲醇-0.4%磷酸溶液(50∶50);检测波长,360nm;流速,0.8ml/min。柱温:35℃。结果:各批全缘绿绒蒿药材TLC色谱中均能检出槲皮素;槲皮素HPLC色谱峰与其它色谱峰分离良好,进样量在0.04304～0.25824μg范围内,呈良好线性关系(r=0.9998),平均回收率为97.14%,RSD=1.24%(n=6)。结论:本法简便快捷,结果可靠,为控制全缘绿绒蒿药材的质量提供参考。

Objective： To establish the TLC identification and quantitative determination methods of Meconopsis integrifplia. Method： TLC and HPLC were adopted to identify and determine Meconopsis integrifplia. The chromatographic conditions were Pbenomenex Luna C18（2）, （4.6 mm× 150 mm, 5μm）column as analytic column, methanol-0.4%H3PO4 water solution （50 i 50） as mobile phase, detection wavelength at 360nm; column temperature at 35 ℃, flow rate at 0.8ml/min. Results： Quercetin was identified by TLC and determined by ttPLC. The liner range of quercetin was 0.043 0400.258 24μg, r - 0.999 8, The average recovery was 97.14%, RSD-1.24%（n=6）. Conclusion： These methods are simple, accurate, reproducible and can be used to enhance the quality control of this drug.