内皮素-1和一氧化氮在肾缺血再灌注损伤时的动态变化及川芎嗪的干预作用

Effect of ligustrazine on the dynamic change of endothelin-1 and nitric oxide during renal ischemia reperfusion injury in rabbits

目的:观测兔肾缺血再灌注损伤时内皮素-1(ET-1)和一氧化氮(NO)的动态变化,探讨川芎嗪注射液的干预作用及其机制。方法:日本大耳白兔30只,随机均分为假手术对照组(S组)、缺血再灌注组(IR组)和缺血再灌注+川芎嗪注射液组(LZ组)。复制在体兔急性肾缺血再灌注损伤动物模型,在缺血前、缺血1 h、再灌注1 h、3 h和5 h分别经颈总动脉抽血检测ET-1和NO含量;实验结束时取肾组织检测ET-1和NO含量。结果:IR组和LZ组血浆ET-1含量均随缺血和再灌注时间的延长呈阶梯性逐渐升高(均P<0.01),而LZ组缺血和再灌注各时点均显著低于IR组(均P<0.01);IR组缺血和再灌注各时点血浆NO含量均明显低于S组(均P<0.01),LZ组缺血和再灌注各时点均显著高于IR组(均P<0.01)。与S组相比,IR组肾组织ET-1含量明显升高,NO含量显著降低(均P<0.01);LZ组ET-1含量差异无显著性(P>0.05),而NO含量明显降低(P<0.05)。与IR组相比,LZ组肾组织ET-1含量显著降低,NO含量明显升高(均P<0.01)。结论:川芎嗪能抑制肾缺血再灌注损伤时肾组织过度产生ET-1,并能使NO分泌增加。

ObJective： To investigate the dynamic change of endothelin-1 （ET-1） and nitric oxide （NO） during renal ischemia-reperfusion injury and to determine the effects of ligustrazine injection on renal ischemia-reperfusion injury and its mechanisms. Methods： Thirty rabbits were divided into three groups randomly： sham operation group （S group）, ischemia reperfusion injury group （IR group） and ischemia-reperfusion injury plus ligustrazine injection group （LZ group）, each group consisted of ten rabbits. The renal ischomia-reperfusion injury model of rabbit was constituted in vivo. The blood plasma gathered from common carotid artery at times： pre-ischemia, ischomia 1 h, reperfusion 1 h, 3 h and 5 h were tested for the content of ET-1 and NO. The kidney tissue sampled at the end of experiment was assayed for the content of ET-1 and NO. Results： The content of ET-1 in plasma was shown a time-dependent ascension in both IR group and LZ group after ischemia-reperfusion （all P〈0.01）,however,it was significant lower in LZ group compared with IR group at same time point during ischemia-reperfusion （all P〈0.01）. The content of NO of plasma at each time point in IR group during ischemia-reperfusion were significant lower than that in S group （all P〈0.01）, and it was significant higher in LZ group compared with IR group at the same time point during ischemia-reperfusion （all P〈0.01）. As compared with S group, content of ET-1 in kidney tissue was increased remarkably, content of NO in kidney tissue was decreased significantly in IR group （all P〈0.01）; while, content of ET-1 in kidney tissue bad not any significant difference （P〉0.05）, content of NO in kidney tissue was decreased significantly in LZ group （P〈0.05）. For LZ group, as compared with IR group, kidney tissue content of ET-1 decreased significantly, content of NO elevated remarkably （all P〈0.01）. Conclusion： Ligustrazine can suppress ET-1 overproduction and increase NO release from kidney tissue during renal ischemia reperfusion injury.