摘要
目的:观察低氧二周对大鼠血浆与心肺组织中新的小分子生物活性肽intermedin/adrenomedullin2(IMD/ ADM2)表达的影响,以探讨其在低氧性肺动脉高压发生发展中的意义。方法:清洁级雄性SD大鼠20只随机均分成对照组和低氧组。采用放免法检测血浆、右心室与肺组织匀浆中IMD/ADM2及其横向同源物-肾上腺髓质素(ADM)的含量,RT-PCR法检测右心室与肺组织中IMD/ADM2及ADM mRNA水平。结果:①低氧组大鼠平均肺动脉压、右心室与左心室加室间隔重量比[RV/(LV+S)]显著高于对照组(P<0.01);②低氧组大鼠血浆中IMD/ADM2与ADM含量均明显高于对照组(P<0.01);③低氧组大鼠右心室与肺组织中ADM含量显著高于对照组(P<0.01),但IMD/ADM2两组间无统计学差别;④低氧组大鼠右心室与肺组织中IMD/ADM2和ADM mRNA水平均显著高于对照组(P<0.05)。结论:在大鼠低氧性肺动脉高压的形成过程中的早中期阶段,血浆、右心室与肺组织中IMD/ADM2蛋白与基因的表达存在差异性。
Airn： To study the effect and significances of two-week hypoxia on the expression of intermedin/adrenomedullin2（IMD/ADM2） in plasma and the tissues of heart and lung in rats. Methods： Twenty male SD rats were randomly divided into normal control group and hypoxia group. The concentrations of IMD/ADM2 and adrenomedullin（ADM） in plasma, right ventricle and lung tissue were measured by radioimmunoassay. RT-PCR was used to detect the mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissue. Results： （1）The mean pulmonary arterial pressure（mPAP） and the weight ratio of right ventricle（RV） to left ventricle plus septum（LV ＋ S） of hypoxia group were significantly higher than those of normal control group（P 〈 0.01 ）. （2）The concentrations of IMD/ADM2 and ADM in plasma were significantly higher in hypoxia group, compared with normal control group （P 〈 0.01 ）. （3）The concentration of ADM in right ventricle and lung tissue in hypoxia group was significantly higher than that in normal control group （P〈 0.01）, while there was no significant difference in IMD/ADM2 between the two groups. （4）The mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissues were significantly up-regulated in hypoxia group（ P 〈 0.05）. Conclusion： The expressions of IMD/ADM2 peptides and gene in plasma, right ventricular and pulmonary tissues are different in the early-middle pathological proceeding of pulmonary hypertension induced by two-week hypoxia in rats.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第1期8-11,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(30871031)
浙江省自然科学基金资助项目(Y207509)
温州市科技局资助项目(Y20070071)
温州医学院重大项目(XNK07006)
