慢性间歇低氧对幼鼠脑区p38MAPK的影响

THE EFFECT OF CHRONIC INTERMITTENT HYPOXIA ON p38MAPK IN CEREBRAL TISSUES OF WEANLING RATS

目的:研究慢性间歇低氧对幼鼠部分脑区p38MAPK的影响。方法:SPF级健康雄性SD幼鼠(3～4周龄)50只,随机分为5组(n=10):间歇低氧2周组(2IH组)、间歇低氧4周组(4IH组)、间歇低氧4周后恢复组(4F组)、对照2周组(2C组)和对照4周组(4C组)。建立慢性间歇低氧幼鼠模型,以RT-PCR法和Western blot法分别测幼鼠海马及前额叶皮层p38MAPK mRNA和磷酸化p38MAPK(p-p38)蛋白的表达。结果:2IH、4IH和4F组幼鼠海马、前额叶皮层的p38MAPK mRNA及p-p38蛋白均明显高于相应对照组(P均<0.05)。结论:慢性间歇低氧可激活幼鼠部分脑区p38MAPK。

Aim： To study the effect of chronic intermittent hypoxia an p38MAPK in partial cerebral tissues of weanling rats. Methods： Randomly, fifty male SD rats（3-week-old- 4-week-old） were divided into five groups： 2-week-CIH group（2IH）, 4-week-CIH group（4IH）, 4-week-recovery group（4F）, 2-week-control group（2C） and 4-week-control group（4C）. Intermittent hypoxia model was induced by an intermittent hypoxia cabin. The expression of p38MAPK mRNA and the phosphorylation levels of p38MAPK（p-p38 protein） in the hippocampus and prefrontal cortex were measured by RT-PCR or Western blot respectively. Results： No matter in the hippocampus, or in the prefrontal cortex, the expression of p38MAPK mRNA and p-p38 protein in 2IH, 4IH and 4F groups were respectively higher than 2C and 4C groups（ P 〈 0.05, respectively）. Conclusion： Chronic intermittent hypoxia can activate the p38MAPK in partial cerebral tissues of weanling rats.