高效液相色谱法测定苹果汁中8种酚类化合物

HPLC Determination of 8 Phenolic Compounds in Apple Juice

提出了高效液相色谱法同时测定苹果汁中儿茶素、绿原酸、咖啡酸、表儿茶素、香豆酸、阿魏酸、槲皮素和根皮素等8种酚类化合物的方法，并考察了光照、保存时间等因素对酚类化合物的稳定性的影响。选用Eclipse XDB C18色谱柱（150mm×4．6mm，5um），以甲醇和乙酸-水（1＋99）溶液作为流动相进行梯度洗脱，利用二极管阵列检测器，在各种酚类物质的最大吸收波长下进行紫外光度检测。对各化合物工作曲线的线性范围作了试验，并推导得到相应的线性回归方程，其相关系数均为0．9999，在苹果汁样品的基础上用标准加入法各进行7次测定，测得平均回收率均在96．0％～100．8％之间，相对标准偏差（n=7）小于等于3．0％，8种化合物的检出限（3S／N）分别为20，10，4，20，5，8，30和7ug·L^-1。

RP-HPLC was applied to the determination of 8 phenolic compounds （including catechin, chlorogenic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, quercetin and phloretin） in apple juice. Effects of illumination and time of storage on the stability of each of the 8 phenolic compounds were studied scrutinously. The Eclipse XDB C18 （150 min× 4. 6 mm, 5 um） chromatographic column was used as the stationary phase. Mixed solutions of methanol and HOAc-H2O solution （1＋99） in different ratio were used as mobile phase in the gradient elution. UV-detections were made at wavelengths of absorption maxima of each phenolic compound by using electrophotodiode array detector. Linearity ranges of the working curves were tested and their linear regression equations, with correlation coefficients of 0. 999 9, were derived. Detection limits （3S/N） of the 8 compounds found were 20, 10, 4, 20, 5, 8, 30 and 7 ug · L^-1 in respect to the order mentioned above. Test for recovery was made by the standard addition method on an apple juice sample for 7 determinations, values of av. recovery found were in the range of 96.0%-100. 8%. Values of RSD＇s （n= 7） obtained from the analytical results were≤ 43.0 %.