摘要
300只1日龄艾维茵肉鸡健雏随机分为5组,分别喂以基础日粮(日粮Se含量0.2 mg.kg-1,对照组)和高硒日粮(Se 1 mg.kg-1,高硒Ⅰ组;Se 5 mg.kg-1,高硒Ⅱ组;Se 10 mg.kg-1,高硒Ⅲ组;Se 15 mg.kg-1,高硒Ⅳ组)6周,用流式细胞术和MTT法研究高硒对外周血T淋巴细胞亚群、淋巴细胞增殖功能的影响。结果显示,当雏鸡日粮硒水平达5 mg.kg-1以上时,外周血CD3+T细胞、CD3+CD4+T细胞和CD3+CD8+T细胞百分比不同程度地降低;高硒Ⅲ、Ⅳ组的T淋巴细胞增殖能力下降,血清IL-2含量降低,与对照组间差异显著或极显著(P<0.05或P<0.01)。结果表明,5 mg.kg-1以上的日粮硒降低雏鸡细胞免疫功能。
By the methods of FCM and MTT, the subsets and lymphocyte proliferation function of peripheral blood T lymphocyte were studied in AA poultries by feeding with sufficient （0.2 mg·kg^-1, control group）, or excess selenium （1, 5, 10, and 15 mg·kg^-1）, as sodium selenite in the diets to five groups of 60 chickens each for 6 weeks. The results showed that the percentage of CD3^＋ T cells, CD3^＋ CD4^＋ T cells and CD3^＋ CD8^＋ T cells were decreased variably in chickens when dietary selenium exceeded 5 mg · kg^-1. The mitogenic response of T lymphocytes to ConA and the content of serum IL-2 were decreased in high Se group Ⅲ and high Se group IV （P 〈0.05 or P〈0.01） when compared with control group. The results suggested that the dietary selenium in excess of 5 mg ·kg^-1 impaired the cellular immune function in chickens.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第2期256-260,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省教育厅重点项目(2006A007)
关键词
高硒
淋巴细胞增殖
淋巴细胞亚群
IL-2
雏鸡
high dietary selenium
lymphocyte proliferation
subset of lymphocyte
IL-2
chicken
