糖基化终末产物上调大鼠心脏微血管内皮细胞NF-κB和COX-2表达

Advanced glycation end products (AGEs) up-regulate the expressions of nuclear factor-kB and cyclooxygenase-2 in rat cardiac microvascular endothelial cells(CMECs)

目的研究糖基化终末产物(AGEs)对大鼠心脏微血管内皮细胞因子κB(NF-κB)和环氧合酶2(COX-2)表达的影响,探讨AGEs与糖尿病血管病变间的关系。方法体外培养大鼠心脏微血管内皮细胞至亚融和状态时,以不同浓度糖基化白蛋白BSAAGEs与之作用不同时间后,检测内皮细胞NF-κB及COX-2蛋白的表达。结果 25、50、100、200mg/L BSA-AGEs作用后,NF-κB和COX-2蛋白表达呈剂量依赖性增多,100mg/LAGEs作用6、12、24、48h,NF-κB和COX-2蛋白表达呈时间依赖性增多。结论 BSA-AGEs可促进体外培养微血管内皮细胞NF-κB和COX-2的表达,其作用可能与NF-κB的活化有关。

Objective To investigate the effects of AGEs on expressions of NF-kB and cyclooxygenase-2 in CMECs. Methods BSA-AGEs was prepared by incubating bovine serum albumin （BSA）with glucose. When CMECs reached a sub-confluence, the stimulating factors were added. The protein expressions of NF-kB and COX-2 were studied by Western blot. Results After treatment with BSA-AGEs in varying concentrations of 25,50,100,200mg/L, the protein expressions of NF-kB and COX- 2 were increased in a dose-dependent manner. Compared with control group, the protein expression of NF-kB was 1.99-, 2.76-, 3.57-, and 4.25-folds rise respectively （n= 5 ,all P〈0.05）; the protein expression of COX-2 was 2. 39-, 3. 41-, 4.49-, 5.34 fold rise respectively （n=5,P〈0.05）. After treatment with AGEs of the same concentration but at varying time of 6,12,24,48h, the protein expressions of NF-kB and COX-2 were increased in a time-dependent manner. Compared with control group, the protein expressions were 1.83-,2.66-, 3.52-, and 4.14-folds rise respectively for NF-kB （n=5 ,P〈0. 05） ,and 2.18-, 3.21-, 4.33-, 4.99-folds rise respectively （n=5,P〈0. 05） for COX-2. Conclusions BSA-AGEs can activate the NF-kB and COX-2 in a dose-dependent and time-dependent manners.