高效液相色谱法测定大鼠血浆中N-去甲氧羰基代谢物(DCJW)的浓度

HPLC determination of decarbomethoxyllated JW062 (DCJW) in rat plasma

目的:建立测定大鼠血浆中N-去甲氧羰基代谢物(DCJW)的高效液相色谱法。方法:以乙腈为沉淀剂处理大鼠血浆后,用反相高效液相色谱-紫外法测定。色谱柱为Shim-pack VP-ODS(150 mm×4.6 mm,5μm);柱温:室温;流动相为乙腈-水(80∶20);流速1.0 mL·min-1;检测波长314 nm。结果:N-去甲氧羰基代谢物(DCJW)的线性范围为0.025～5.00μg·mL-1,r=0.9998;最低检测限为0.020μg·mL-1。血浆中DCJW的绝对回收率为97.0%～100.5%,方法回收率为95.2%～101.0%,日内和日间RSD均小于8.0%。结论:本法专属性强,灵敏度高,重复性好,适用于DCJW血药浓度测定及药代动力学研究。

Objective： To develop an HPLC - UV method for determining of decarbomethoxyllated JW062 （DCJW） concentration in rat plasma. Method：The plasma samples were de -proteinized with acetonitrile in a one -step ex- traction. The HPLC assay was carried out using a Shim - pack VP - ODS（ 150 mm ×4. 6 mm ,5 μm） column and a mo- bile phase consisting of aeetonitrile - water （80：20 ） at a flow rate of 1.0 mL ·min^ - 1. The detection wavelength of the UV absorption detector was set at 314 nm. Results：The method was fully validated with the following validation pa- rameters： linearity range 0. 025 - 5. 00μg· mL^-1 （ r 〉 0. 999 ） ; limit of detection 0. 020 μg· mL^ -1. Absolute recoveries of DCJW were 97.0% - 100. 5% from rat plasma;method recoveries were 95. 2% - 101.0%. Both of intra - and inter-day RSD were less than 8.0%. Conclnsions：The method is sensitive, accurate and reproducible,which is suit-able for the determination of DCJW in rat plasma. This method can be used in the study of pharmacokineties.