摘要
目的改进乳块消片中丹参素钠含量测定的提取方法,优化测定条件。方法采用反相高效液相色谱法,选用Ultimate AQ-C18色谱柱,流动相为乙腈-甲醇-水-冰醋酸（0.5∶8∶92∶1）,流速为1.0mL·min^-1,检测波长为280nm。结果丹参素钠在0.0950-0.8550μg·mL^-1范围内呈良好线性,r=0.9998,方法平均回收率为100.21%,RSD=0.49%。结论改进原有提取方法后操作简便、快速,结果准确,分离效果好,为乳块消片的质量评价提供了依据。
Objective To establish a HPLC method for improving the way of extracting for determination condition and optimization quantitative determination. Methods Ultimate AQ-C18 column was used with acetonitrile-methanol-water-icy acetic acid （0. 5 ： 8 ： 92 ： 1） . The flow rate 1.0mL · min^-1 and the detection wavelength was 280nm. Results The sodium danshensu presents a good Linearity （r = 0. 9998） within the range of 0. 0950-0. 8550μg ·mL^-1. The average recovery rate were 100. 21% and the RSD=0.49% （n= 6） . Conclusions The Improved method of extracting is simple, fast, accurate and with a good separating result. Thus provide a basis for the quality evaluation of the Rukuaixiao Tablets.
出处
《中国药事》
CAS
2009年第2期162-164,共3页
Chinese Pharmaceutical Affairs
关键词
乳块消片
丹参素钠
HPLC
提取条件
含量测定
Rukuaixiao Tablets
soldium danshensu
HPLC
extracting condition
quantitative determination
