蜂蜡素对HepG_2细胞HMG-CoA还原酶mRNA表达及蛋白质水平的影响

Effect of Fenglasu on the expression of HMG-CoA reductase mRNA and protein in HepG_2 cells

目的:探讨蜂蜡素对HepG2细胞HMG-CoA还原酶mRNA表达及蛋白质水平的影响。方法:采用MTT比色法,Western Blot,RT-PCR等技术方法,观察蜂蜡素对HepG2细胞存活能力、HMG-CoA还原酶mRNA表达及蛋白质水平的影响。结果:当蜂蜡素浓度为100mg·L-1或以下时,对细胞存活能力无影响,为本研究可选择的剂量范围;蜂蜡素处理HepG2细胞,随着蜂蜡素浓度升高(低、中、高浓度为0.8,8,80mg·L-1),HMG-CoA还原酶蛋白质水平和mRNA的表达均逐渐降低,呈现明显的浓度依赖关系。结论:蜂蜡素可抑制HepG2细胞HMG-CoA还原酶蛋白质及mRNA的表达。

Objective： To investigate the effect of Fenglasu on the expression of 3-hydroxy-3-methylglutaryl-eoenzyme A （HMG-CoA） reductase mRNA and protein in HepG2 cells. Methods： The cell proliferation was determined by MTT assay. The expression of HMG-CoA reductase mRNA and protein was determined by RT-PCR and Western blot. Results：As indicated by MTT assay, Fenglasu at 100 mg·L^-1 had no cytotoxicity in HepG2 cells, which provided the safe range for the following experiments. Both results of RT-PCR and Western blot showed that the mRNA and protein expression of HMG-CoA reductase gradually decreased with the increase of Fenglasu concentration （0.8, 8 and 80 mg·L^- 1 ）. Conclusion ： Fenglasu can inhibit the expression of HMG-CoA reductase mRNA and protein in HepG2 cells.