正交试验优化提取板蓝根药材中核苷类成分的研究

Studies on extraction process of nucleosides for radix isatidis

目的:优化板蓝根提取工艺条件。方法:以尿苷、鸟苷、腺苷的总含量为指标,采用正交试验对板蓝根水提工艺的加水量、回流次数、回流时间进行研究。结果:最佳提取条件为:用6倍量水回流提取3次,每次1h,上述3种核苷转移率为90.02%。结论:本工艺3种核苷总量的提取转移率较高,且工艺稳定性好,重现性好。

Objective ： To optimize the extraction process for radix isatidis. Methods ： The process was studied by orthogonal design with the total content of guanosine, uridine, and adenosine as the detective markers. Resuits：The optimized extraction condition was obtained. The crude material was extracted 3 times with 6 times volume of water for 1 h. The extraction rate of the three nucleosides was 90.02% in the process above. Conclusion： The high extraction rate of nucleosides was obtained with high stability and reproducibility.