淫羊藿苷糖苷酶的纯化及其酶学性质的研究

Purification of Icariin-glycosidase and Its Enzymatic Characteristics

对由菌株Absidiasp.E9r在发酵培养中产生的淫羊藿苷糖苷酶进行了分离纯化并对其酶性质进行了研究。结果表明,该酶的分子质量约为65ku,酶反应的最适温度和pH值分别为50℃和4.0,在20～60℃,pH3.0～6.0条件下稳定性较好;金属离子Na+、K+、Mg2+、Zn2+、Ca2+对酶反应的影响不大,而Fe3+、Cu2+对其有很明显的抑制作用;酶反应动力学研究,Vmax=3.012mmol/(L.h),km=58.59mmol/L。

The Icariin--glycosidase which produced by a strain named Absidia sp. E9r was purified and part of its characteristics were studied in this paper. The result indicated that its molecular weight is about 65 ku. The optimal temperature and pH for enzyme reaction were 50℃ and 4.0 respectively. Under the conditions of temperature 20-60℃,pH 3.0-6.0,the enzyme is stable. Na^＋,K^＋,Ca^2＋,Mg^2＋,Zn^2＋ ions had no effect on the enzyme activity, while Fe^3＋ ,Cu^2＋ inhibited the enzyme activity. The Vmax and Km of the enzyme are 3. 012mmol/（L·h） and 58.59mmol/L.