核桃蛋白肽的抗氧化活性研究

Study on the Antioxidative Activity of Walnut Peptide

以VC为对照,研究了分子质量小于3000u的核桃蛋白肽混合物的还原能力、羟自由基清除能力、超氧阴离子自由基清除能力以及二苯代苦味酰基自由基清除能力。结果显示,随着核桃蛋白肽浓度的增加,其还原能力、羟自由基的清除能力和超氧阴离子清除能力增大,在浓度为30mg/mL时,核桃蛋白肽还原能力达到VC的57.1%;在浓度为50mg/mL时,核桃蛋白肽对羟自由基(OH.)的清除率分别69.1%,对超氧阴离子自由基的清除率达到了85%。而对二苯代苦味酰基自由基清除能力则是先增大后变小,在20mg/mL时达到最大值66%。核桃蛋白肽的分子质量分布主要有2大区域,蛋白肽氨基酸含量达到89.74%。

The reducing ability and hydroxyl, superoxide radical--scavenging activity and DPPH ·scavenging activity of walnut peptides whose molecular weight is lower than 3000 were observed, and compared with those of vitamin C. The results showed that the reducing ability and hydroxyl, superoxide radical-- scavenging activity were increased with the concentration of peptides. At 30 mg/mL, the ability of peptides was 57.1% of vitamin C. At 50mg/mL, hydroxyl, superoxide radical--scavenging activity were 69.1% and 85.0% respectively. The results also showed that DPPH · scavenging activity was first increased and then decreased with the concentration of peptides, and at 20mg/mL, DPPH · scavenging activity reached its peak with the value of 66%. The molecular weight distribution of the peptide contained two main region and the amino acid of the peptide were 89.74%.