活性炭吸附分离芳香族氨基酸的研究

Study on the Absorption and Desorption of Aromatic Amino Acids with Active Carbon

采用动态吸附法,对18种活性炭进行了筛选,并确定吸附条件为pH3,吸附温度25℃,炭-液比1∶12,吸附时间120min。根据活性炭吸附前后的蛋清寡肽混合物溶液的全波长紫外扫面结果,发现活性炭对芳香族氨基酸吸附作用较强。经氨基酸自动分析仪测得蛋清高F值寡肽溶液F值为22,游离氨基酸含量7.8%,凝胶过滤色谱测定其分子质量分布为连续的,绝大部分分布在300～800u。

The absorption of eighteen active carbon was investigated. The optimal active carbon was determined and its absorption was studied. The optimal conditions were： No. 4 active carbon, used to remove free aromatic amino acids by the method of static absorption; the weight of activated carbon to the volume of hydrolysate ratio 1 ： 12; the temperature was 25℃, pH3; the time was 120min. The result of UV-scanning indicated that active carbon was efficient to absorb aromatic amino acids. The amino acids composition of the Fischer ratio of egg white oligo-peptides was studied. G-25 gel filtration chromatography was used to determine the molecular weight of the oligo-peptied. The molecular weight ranged within 300-800.